GEM基因在3T_3-L_1脂肪细胞诱导分化过程中的表达变化

Changes of GEM Gene Expression During 3T_3-L_1 Preadipocyte Differentiation

目的观察3T3L1前体脂肪细胞诱导分化过程中GEM基因表达水平的变化,探讨GEM基因与肥胖发生的关系。方法体外培养3T3L1前体脂肪细胞,采用RTPCR技术检测分化不同时段的3T3L1脂肪细胞中GEM基因的mRNA表达水平。结果在3T3L1脂肪细胞的诱导分化过程中,GEM基因mRNA表达水平无显著变化(P>0.05)。结论GEM基因在脂肪细胞分化、脂质合成和积聚过程中的作用有待进一步研究。

Objective To investigate the changes of GEM gene expression during 3T3-L1 preadipocyte differentiation and to explore the relationship between GEM gene and the etiology of obesity. Method 3T3-L1 preadipocytes were cultured in vitro and differentiated into the matured adipocytes. Total RNA was extracted from adipoeytes of various times and the levels of GEM gene mRNA expression were evaluated by RT-PCR. Results During the differentiation of 3T3-L1 preadipocytes,the expression level of GEM gene mRNA didn＇t change significanfly（P 〉0.05）. Conclusion Further studies may be needed to explore the role of GEM gene in 3T3-L1 preadipocyte differentiation as wells as lipid synthesis and accumulation.