香叶天竺葵的组织培养快速繁殖

In Vitro Promot Proliferation of Pelargonium graveolens

以香叶天竺葵的叶片和叶柄切段为外植体诱导愈伤组织,经过芽诱导、生根诱导等过程成功获得了香叶天竺葵的再生植株。对香叶天竺葵芽的诱导、芽的继代增殖、根的诱导及再生植株移栽等环节进行了优化,在对比研究过的各种培养基中,MS+0.2mg·L-1NAA+0.75mg·L-1BA为最适宜的芽诱导和增殖培养基,芽诱导率达30%,不定芽增殖频率也高于其它培养基;1/2MS+0.2mg·L-1NAA最适于进行生根诱导,生根率高达92%,在该培养基中诱导生根,再生植株的根和芽均显示出较好的长势,移栽成活率也高。该项研究为香叶天竺葵的工厂化大规模育苗提供了依据。

Leaf and stalk segments of Pelargonium graveolens as explants were cultured to induce callus and then through the process of inducing bud and root clonal propagation of Pelargonium graveolens was successfully developed in vitro. Several procedures, such as inducement and multiplication of lush buds, cultivation of growing roots and transplanting, were optimized. Comparing with other media involved the MS ＋ NAA0.2mg · L^-1 ＋ BA0. 75mg · L^-1 was appropriate for the inducing and multiplying of lush buds. 1/2MS ＋ NAA0.2mg · L^-1 had a better effect on inducing roots with 92% growing roots percengtage and higher survival percentage of transplanting. Our study may provide basis for raising seedling in a factory on a large scale.