摘要
以能量转移体系作为DNA荧光探针是测定DNA的新方法,该方法灵敏、快捷、重现性好,其DNA的线性范围为0.067~11.73 μg·mL-1,检出限为0.054 μg·mL-1,对0.80 μg·mL-1的DNA进行了10次测定,相对标准偏差为0.49%.该荧光探针用于混合样品的测定,结果令人满意.
It is a good method to use energy transfer system as DNA fluorescent probe to determine DNA. The method is sensitive , rapid and reproducible. Linearity is observed in the range 0. 067~11.73μg·mL^-1 ,the limit of detection is 0. 054μg·mL^-1,the relative standard deviation (RSD) is 0. 49% for the determination of 0. 8μg·mL^-1 DNA(n= 10). The fluorescent probe is applied to the determination of DNA in mixed sample and achieves satisfactory result.
出处
《安庆师范学院学报(自然科学版)》
2005年第3期67-69,共3页
Journal of Anqing Teachers College(Natural Science Edition)