荧光素—酚藏花红能量转移体系用于测定DNA

The Application of Fluorescein-phenosafranine Energy Transter System to the Determination of DNA

以能量转移体系作为DNA荧光探针是测定DNA的新方法,该方法灵敏、快捷、重现性好,其DNA的线性范围为0.067～11.73 μg·mL-1,检出限为0.054 μg·mL-1,对0.80 μg·mL-1的DNA进行了10次测定,相对标准偏差为0.49%.该荧光探针用于混合样品的测定,结果令人满意.

It is a good method to use energy transfer system as DNA fluorescent probe to determine DNA. The method is sensitive , rapid and reproducible. Linearity is observed in the range 0. 067～11.73μg·mL^-1 ,the limit of detection is 0. 054μg·mL^-1,the relative standard deviation （RSD） is 0. 49% for the determination of 0. 8μg·mL^-1 DNA（n= 10）. The fluorescent probe is applied to the determination of DNA in mixed sample and achieves satisfactory result.