摘要
目的研究溶解红细胞对脑出血后脑水肿的影响。方法大鼠48只,随机分为3组:A组:生理盐水组,利用立体定向仪向大鼠右侧尾状核注入生理盐水50μl;B组:自体全血组,大鼠右侧尾状核注射自体全血50μl;C组:溶解红细胞组,大鼠右侧尾状核注射自体溶解红细胞50μl。用干湿重法、比色法、免疫组化法分别测定脑损伤程度及脑水肿程度。结果溶解红细胞组脑含水量:(82·3±1·84)%高于生理盐水组(77·5±1·55)%(P<0·05);溶解红细胞组脑伊文斯兰含量:(13·86±1·38)μg/g、HO-l染色阳性细胞数(17·88±5·30)个,均高于生理盐水组(4·43±0·86)μg/g、(0·38±0·52)个和自体全血组(11·76±1·15)μg/g、(9·75±2·82)个(P<0·05)。结论溶解红细胞在脑出血后脑水肿的形成中具有重要作用,相同时间内溶解红细胞造成的脑水肿大于自体全血。
Objective To study the effect of lysed erythrocyte on brain edema after rat intracerebral hemorrhage.Methods 48 healthy Wistar rats were distributed to 3 groups randomly, using stereotactic guidance to group A： infuse saline 50μl into the right caudate; group B： infuse rat self- body - blood 50 μl into the right caudate; group C： infuse antotolgous lysed erythrocyte 50μl into the right caudate; using dry - raw weighted method and immunhistochemical ABC raethod to observe degree of brain injury, and brain edema. Results The results of brain water content in group C is higher than group A, （ P 〈 0.05）, the means of Evans blue contents and HO - 1 positive ceils in group C is higher ttran other groups, （ P 〈 0.05） . Conclusion Antologous lysed erythrocyte plays an important role in brain edema formation following ICH, the injury degree of lysed erythrocytes is more severe than selfbody blood.
关键词
溶解红细胞
大鼠
脑出血
脑水肿
血红素加氧酶
Intracerebral hemorrhage
Brain edema
Lysed erythrocyte
Heme oxygenase- 1 （HO- 1）
