电针对抑郁模型大鼠行为学的影响

Effect of Electroacupuncture on Changes of Behavior in Depression Rats

目的:通过观察电针对抑郁模型大鼠行为的影响,明确针刺治疗抑郁症的作用,并为进一步的机制研究提供依据。方法:30只SD大鼠随机分为正常组、模型组和电针组,每组10只。用慢性不可预见性轻度刺激结合孤养的方法造模,给予模型组和电针组21 d不同的应激刺激。造模同时针刺治疗电针组动物,选取“百会”“印堂”穴,频率2 Hz,电流强度为0.6 mA左右,留针20 min,每日1次,连续针刺21 d。于实验前1 d、实验第7、142、1 d测量大鼠体重,并进行开野实验及糖水消耗试验。结果:造模7 d后模型组的体重、水平运动和垂直运动即明显低于正常组(P<0.05,0.01,0.001);电针组的体重在第7 d也出现了减少(P<0.001),与模型组一直未见差异,但水平运动与垂直运动次数始终高于模型组(P<0.05,0.01,0.001),与正常组未见差异。第21 d的糖水消耗试验显示,模型组相对糖水消耗量低于正常组和电针组(P<0.05),电针组与正常组之间无显著差异。结论:慢性不可预见性轻度刺激结合孤养的方式能造成大鼠中枢奖赏系统的功能低下,电针能够防治由此引发的大鼠行为学的改变。

Objective： To observe the effect of electroacupuncture （EA） on changes of behavior in depression rats. Methods： Thirty male SD rats were evenly randomized into control, model and EA groups. Depression model was established by using chronic unpredictable mild stress （CUMS） stimulation （forced ice-water swimming, electric shock, tail-clamping, etc） combined with lonely raising for 21 days. EA （2 Hz, 0.6 mA, 20 min） was applied to “Baihui” （GV 20） and “Yintang”（EX-HN 3）, once daily, continuously for 21 days. The rat＇s body weight, open-field test, absolute and relative volume of sucrose-intake were recorded one day before experiments, and on the 7^th, 14^th and 21^st day during experiments respectively. Results： Compared with control group, the rats＇ body weight, crossing and rearing motion times and sucrose-intake volume decreased significantly in model group （ P〈0.05,0.01,0.001 ）. From the 7^th to 21^st day, the body weight of both model and EA groups was significantly lower than that of control group （ P〈0. 001 ）, but no significant difference was found between model and EA groups. Compared with control group, the crossing and rearing movement times on the 7^th and 21^st day, the absolute volume of sucrose-intake on the 14^th and 21^st day, and the relative volume of sucrose-intake on the 21^st day of model group decreased significantly （ P〈 0.05,0.01, 0. 001 ）. In comparison with model group, the rats＇ crossing and rearing movement times from 7^th to 21^st day, absolute and relative volume of sucrose-intake on the 21^st day increased considerably in EA group （P〈 0.05,0.01,0.001 ）. The results suggested that EA could prevent or suppress the onset of depression and raise the volume of sucrose-intake in depression rats. Conclusion： combined with lonely breeding may cause a hypofunction of the rat＇s central reward system, whereas EA treatment can prevent the rat＇s abnormal behavior.