泽泻rDNA ITS区序列特征及其居群鉴别研究

Studies on Characteristics of rDNA ITS Sequences and Population Authentication of Alisma orientale

运用PCR产物直接测序法对川泽泻、建泽泻和江泽泻的rDNA ITS区(包括ITS1,5.8S,ITS2)碱基序列进行了序列测定和分析.泽泻rDNA ITS区的碱基序列总长度确定为640 bp,江泽泻和建泽泻的ITS区碱基序列完全一致,川泽泻与建泽泻(江泽泻)在rDNA ITS区碱基序列有2个稳定的变异位点,分别位于ITS1和ITS2区段.我国的泽泻与意大利泽泻在5.8S保守区(第289位)有一个碱基差异.依据泽泻rDNA ITS区的序列特征可以进一步鉴别川泽泻和建泽泻,为泽泻居群的鉴别提供可靠的分子标记.

rDNA ITS regions （ including ITS1, 5.8 S and ITS2 ） of Alisma orientale from Sichuan , Fujian and Jiangxi provinces were sequenced by PCR products sequencing method and analyzed. The sequences of rDNA ITS region of Alisma orientale ranged 640 bp. The sequence of Alisma orientale from Jiangxi and Fujian provinces was the same , and there were 2 stable variable sites among Alisma orientale populations which were collected from Sichuan and Fujian（Jiangxi） provinces. One was in ITS-1, and the other was in ITS-2. There was another different site in 5.8 S （ No. 289 ） region of rDNA ITS region between Alisma orientale of China and Italy. The difference of rDNA ITS sequences can be used to authenticate accurately the populations of Alisma orientale from Sichuan and Fujian, and to provide reliable molecular markers for identifying Alisma orientale populations.