伤寒沙门菌的脉冲场凝胶电泳分型方法研究

Study on genotyping of Salmonella typhi by pulsed-field gel electrophoresis

目的:运用脉冲场凝胶电泳研究宁波市伤寒沙门菌的分子流行病学。方法:选择XbaⅠ酶对伤寒沙门菌染色体DNA进行酶切,采用脉冲场凝胶电泳技术分析电泳酶切指纹图谱,了解其流行状况。结果:14株伤寒沙门菌,根据电泳图谱分析,可分为3个带型。结论:PFGE具有分辨力高,重复性好的特点,是研究伤寒沙门菌分子流行病学较好的基因分型方法。

Objective ： To study molecular epidemiology of Salmonella typhi in the city by the technique of pulsed - field gel electrophoresis （PFGE）. Methods： The chromosome DNA in Salmonella typhi was digested by Xba Ⅰ. The diagram was obtained by PFGE to know the prevalence of Salmonella typhi Results ： Three different types were found among 14 strains of Salmonella typhi. Conclusion ： PFGE is a better genotyping method to research molecular epidemiology of Salmonella typhi.