摘要
抗酒石酸醚性磷酸酶（ＴＲＡＰ）是破骨细胞（ＯＣ）的标志酶，为ＯＣ所特有。本研究应用改良的ＶａｎＤｅＷｉｊｎｇａｅｒｔ和Ｍｏｓｔａｆａ方法对培养在骨片上的ＯＣ进行ＴＲＡＰ染色，成功地观察了体外培养ＯＣ的形态与生存时间。该方法简便、特异性高，能准确计数体外培养的ＯＣ数，所用底物为萘酚ＡＳ－ＢＩ，原料易获得，培养在骨片上的ＯＣ生存时间可长达２４０小时。
Tartrate resistant acid phosphatase(TRAP)is a marker enzyme of osteoclasts(OC)and specific to the OC,With modified Van De Wijngaert and Mostafa's method f the OC cultivated onbone slices was stained with TRAP, Its morphology and survival period was observed successfully.Thernethod was simple,high specific and could be used accurately to number the OC cultivated in vitro,Thesubstrate being used was naphasol AS-BI which could be obtained easily,The OC cultivated on boneslices could survive as long as 240 hours.
出处
《泰山医学院学报》
CAS
1996年第2期91-93,共3页
Journal of Taishan Medical College
关键词
破骨细胞
细胞培养
生存时间
TRAP
染色
osteoclasts
cell culture
survival period
tartrate resistant acid phosphatase
