甘肃道地药材大黄不同部位的指纹图谱研究

Study on the Fingerprints of Different Parts of Rhubarb from Genuine Medicinal Materials in Gansu Province

目的:考察甘肃道地药材大黄不同部位(根头、根身和根尾)有效成分的差异性,为其质量控制提供理论依据。方法:采用高效液相色谱法对大黄不同部位提取物的指纹图谱进行比较研究,其中色谱柱为C18,流动相为甲醇-0.1%磷酸水溶液(70∶30),流速为1.0ml/min,柱温为室温,检测波长为280nm。结果:大黄不同部位的高效液相色谱指纹图谱具有明显的区别,有效成分大黄素、大黄酚等含量差别很大,含量自根头、根身和根尾部位逐渐递减。结论:为更好地保证大黄药材的药用价值和质量,应区别不同部位对其进行质量控制。

OBJECTIVE： To investigate the differences of the active components of different parts of rhubarb（the head, the body and the end part of the root） from genuine medicinal materials in Gansu province so as to provide theoretical basis for its quality control. METHODS： A comparative study of the fingerprints of different parts of rhubarb extract were conducted by HPLC, in which, the C18 was used as the chromatographic column under room temperature, the mobile phase consisted of menthol 0.1% H3PO4（70：30）with a flow rate of 1.0ml/min, the column＇s temperature of room temperature and detection wavelength of 280nm. RESULTS： Significant differences were noted in the HPLC fingerprints of different parts of rhubarb, there were significant differences in the contents of the active components such as emodin, chrysophanol, etc., the contents decreased progressively from the head of root, the body part to the end part. CONCLUSION： In order to ensure the medical value and the quality of rhubarb medicinal material, different parts of which should be differentiated in the quality control.