生殖器单纯疱疹病毒感染的快速诊断与病毒分型

Rapid diagnosis and typing of genital herpes simplex virus infection

采用细胞培养,短时细胞培养(24h)免疫酶染色及单纯疱疹病毒单克隆抗体(HSV-McAb)酶联免疫吸附试验(ELISA)方法检测了81份不同性病患者的生殖器标本。结果常规细胞培养、短时细胞培养免疫酶染色及 ELISA 的 HSV 刚性检出率分别为39.5%,38.2%和32.1%(x^2=1.096,p>0.05)。与常规细胞培养分离病毒相比,免疫酶染色技术的灵敏性为93.7%,特异性为97.9%,而 ELISA 灵敏性为81.3%,特异性达100%。短时细胞培养免疫酶染色检出单纯疱疹病毒抗原(HSV-Ag)全部阳性,而常规细胞培养分离 HSV 平均需3.5d,最长需时10d。

This paper reports the use of conventiohal cell culture,cell culture plus immunoperoxidase staining and ELISA for detecting the genital samples of 81 patients with venereal diseases.The results showed that the posi- tive rates of conventional cell culture,immunoperoxidase staining and ELISA were 39.5%,38.2% and 32.1%, respectively(P>0.05).The comparison among the results of conventional cell culture with immunoperoxidase stain- ing and ELISA,the sensitivity and specificity of immunoperoxidase staining were 93.7% and 97.9% respectively, and the sensitivity and specificity of the ELISA were 81.3% and 100%,respectively.The immunoperoxidase stain- ing was obtained within 24 h,and the average time of conventional cell culture were 3.5 days,with the longest of 10 days.The immunoperoxidase staining is a sensitive,rapid and specific method for detecting the genital herpes simplex virus infection.