风湿性关节炎与Epstein-Barr病毒关系的研究

Studies on the relationship between rheumatic arthritis and Epstein-Barr(EB)virus

采用抗体检测和聚合酶链反应(PCR)技术,对风湿性关节炎病人血清内抗 Epstein-Barr(EB)病毒核抗原(EBNA),壳抗原(VCA)和早期抗原(EA)的 IgG 抗体,及周围血粒细胞、口腔和病变关节腔积液中脱落细胞内 EB 病毒 BamH Ⅰ K 片段存在情况进行了较系统、全面的研究。实验中发现,病人血清内抗 EB 病毒抗体滴度显著高于正常人。两组人血清中抗 EB 病毒抗体的几何平均滴度之比分别是:EBNA3.1:1,VCA4.0:1,EA4.8:1。在病人和正常人血粒细胞和口腔脱落上皮细胞中分别检出了 EB 病毒 BamH Ⅰ K 240bp 的 DNA 片段。在风湿性关节炎和骨关节炎病人关节滑膜腔积液脱落细胞中也扩增出 EB 病毒的基因片段。对经药物治疗后病人血清内抗 EB 病毒不同成分抗体滴度的变化进行了动态观察,证明药物治疗3个月后,病人血清内抗 EBNA-1抗体滴度下降明显,而IgG/VCA 和 IgG/EA 等抗体的滴度几乎无变化。还对系统性红癍狼疮和系统性硬化症病人血清内抗EB 病毒 IgG 抗体滴度进行了测定。

Rheumatic arthritis(RA)is a high incidence disease in China.Anti EB virus EBNA,VCA and EA antibodies were detectable in the sera from RA,systemic lupus erythematosus(SLE),systemic sclerosis(SS)patients and normal individuals respectively.There is a great difference between the antibody titers from sera of patients with RA and normal individuals.In an immunofluorescence assay for anti-EBNA,anti-VCA and anti-EA antibodies,the ratios of geometric mean titers(GMT)were 3.1:1,4.0:1 and 4.8:1 respectively for those of RA patients to those of the normal individuals.The 240 bp DNAs of EB virus BamHI K fragment were amplified in peripheral granulocytes and desquamated epithelial cells of buccal fluids from patients with RA,SLE,SS and nor- mal individuals,and this 240 bp DNA fragments were also found in desquamated cells of synovial fluids from the RA and Osteoarthrosis(OA)patients respectively.Anti-EB virus EBNA2 and EBNA3 antibodies were also detectable in the sera from RA,non-self-immunity patients and the EBNA1 negative individuals. The anti-EBNA-1 antibody titers decreased rapidly after the treatment to RA patients with drugs,howeve,it was found that there were no change in the IgG/VCA and IgG/EA antibody titers.