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苜蓿花叶病毒复制酶基因全长cDNA植物表达载体的构建

Construction of Plant Expression Vector for The Full Length cDNA of AlMV Replicase Gene
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摘要 将苜蓿花叶病毒中国分离株(A lfalfa m osaic v irus Ch inese iso late,A lMV-Ch)的复制酶P2亚基(90 KD蛋白)基因的全长cDNA构建到植物表达载体pROKⅡ中,得到重组植物表达载体pA lMV-FL. The full length cDNA of the replicase gene which encodes 90 KD protein in AlMV was recombinated with the plant expression vector pROK Ⅱ. Then the recombinated vectors pAlMV-FL was obtained.
出处 《天津师范大学学报(自然科学版)》 CAS 2005年第3期29-31,共3页 Journal of Tianjin Normal University:Natural Science Edition
基金 国家自然科学基金资助项目(39860042)
关键词 苜蓿花叶病毒 复制酶基因 植物表达载体 alfalfa mosaic virus replicase gene plant expression vector
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参考文献5

  • 1Barker R F, Jarvis N P, Thompson D V, et al. Complete nucleotide sequence of alfalfa mosaic virus RNA3[J]. Nucleeic Acids Research, 1983, 11:2881.
  • 2Taschner PEM, Van der Kuyl AC, Neeleman L, et al. Replication of an incomplete alfalfa mosaic virus genome in plants transfromed with viral replicase genes [J]. Virology, 1991,181:445.
  • 3Mori M, Mise K, Okuno T, et al. Expression of brome mosaic virus-encoded replicase genes in transgenic tobacco plants[J]. Journal of General Virology, 1992, 73:169.
  • 4Golemboski D B, Lomonossoff G P, Zaitlin M. Plants transformed with a tobacco mosaic nonstructural gene sequence are resistant to the virus[J]. Proc Acad Sci, USA, 1990, 87:6311.
  • 5张竞秋,哈斯阿古拉,张丽,张鹤龄.苜蓿花叶病毒复制酶基因的克隆、序列分析及其植物表达载体的构建[J].内蒙古大学学报(自然科学版),2003,34(5):536-541. 被引量:5

二级参考文献6

  • 1彭学贤,项瑜,刘俊君,莽克强.马铃薯Y病毒复制酶基因的克隆和序列分析[J].生物多样性,1994,2(1):35-37. 被引量:8
  • 2Mori M ,Mise K, Okuno T,et al. Expression of brome mosaic virus-encoded replicase genesin transgenic tobacco plants [J]. Journal of General Virology, 1992,73 : 169 ~ 172.
  • 3Taschner PEM,Van der Kuyl AC,Neeleman L,et al. Replication of an incomplete alfalfa mosaic virus genome in plants transfromed with viral replicase genes [J]. 1991,181 :445~450.
  • 4Golemboski D B,Lomonossoff G P,Zaitlin M. Plants transformed with a tobacco mosaic nonstructural gene sequence are resistant to the virus [J].Proc Acad Sci USA. 1990,87:6311~6315.
  • 5Cornelissen B J C,Brederode F Th,Veenman G H,et al. Complete nucleotide sequence of alfalfa mosaic virus RNA2 [J]. Nucleic Acid Research, 1983,11 (10) : 3 019~ 3 025.
  • 6任兵,王春香,潘乃穟,陈章良.烟草花叶病毒54 kD基因的cDNA克隆及序列分析[J].Acta Botanica Sinica,1992,34(11):829-834. 被引量:3

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