基因重组干扰素γ对骨髓增生异常综合征

STUDY ON THE TREATMENT OF MYELODYSPLASTIC SYNDROME BY GENE RECOMBINANT INTERFERON γ IN VITRO

用基因重组干扰素（ＩＦＮ－γ）对４１例骨髓增生异常综合征（ＭＤＳ）病人造血祖细胞的作用进行了体外观察，结果：①ＩＦＮ－γ体外对非血液病对照组病人的红系祖细胞（ＣＦＵ－Ｅ）和粒系祖细胞（ＣＦＵ－ＧＭ）无明显促增殖作用，而对ＭＤＳ病人的ＣＦＵ－Ｅ和ＣＦＵ－ＧＭ有明显促增殖作用。其作用呈剂量依赖型，在ＩＵ／ｍｌ时作用最小，１００Ｕ／ｍｌ时作用最强；②ＩＦＮ－γ对ＭＤＳ原ＣＦＵＥ和ＣＦＵ－ＧＭ增殖正常组加入ＩＦＮ－γ后集落数增加最明显，而对增殖明显减低组集落数无明显增加；③ＩＦＮ－γ对ＭＤＳ不同亚型病人ＣＦＵＥ和ＣＦＵ－ＧＭ作用不同，其中对转化中原始细胞增多的难治性贫血（ＲＡＥＢ－Ｔ）型病人有效率较高。

Hematopoietic progenitor cells from 41 patients with myelodysplatic syndrome (MDS) were cultured with gene recombinant interferon γ (IFN γ) in vitro . The results were as follows: (1) In MDS group, IFN γ accelerated greatly the proliferation of CFU E and CFU GM growth, while normal control group, it had no response.This action was dependent on the dosage. Predominant effect was achieved when 100U/ml of was used and the effcet was the smallest when 1U/ml of it was used.(2)The clonal number increased obviously in the MDS group with normal CFU E or CFU GM,and no remarkable increase was found in the MDS group with proliferation decreased. (3) The action of IFN γ was different and dependent on the types of MDS, the growth rate was better in patients with RAEB T.