重组人粒细胞集落刺激因子对照射小鼠造血功能的影响

EFFECT OF rhG CSF ON THE HEMATOPOIETIC REGENERATION OF MICE EXPOSED TO γ RAY IRRADIATION

为研究重组人粒细胞集落刺激因子（ｒｈＧ－ＣＳＦ）对急性放射病的治疗作用，观察了不同剂量的ｒｈＧ－ＣＳＦ对６．５Ｇｙ照射小鼠造血恢复的影响。结果表明，予受照小鼠每日ｒｈＧ·ＣＳＦ１．２５、２．５０及５．００μｇ治疗后，外周血白细胞、红细胞及血小板的恢复明显加快，以５．００Ｖｇ剂量组效果为最佳；照射后１０ｄ骨＊及脾脏ＣＦＵ＋ＧＭ、暴增型红系祖细胞集落（ＢＦＵ－Ｅ）及混合型祖细胞集落（ＣＦＵ·Ｍｉｘ）含量及生成率均明显高于对照组，并具有剂量相关性；内源性脾结节数随给药剂量的加大而增加。表明ｒｈＧ－ＣＳＦ不仅能加速粒系造血恢复，而且对红系及巨核系造血恢复也有明显的促进作用，因而有希望成为放射病治疗药物之一。

In order to study the therapeutic effect of rhG CSF in acute radiation sickness, different doses of rhG CSF (1.25, 2.50 and 5.00 μg/d) were given to 6.5Gy γ ray irradiated C57/BJ mice and its effect on hematopoietic recovery was evaluated. It was shown that peripheral WBC,RBC and platelet counts recovered much quicker in the mice treated with the factor; the bigger was the dose of rhG CSF, the quicker did the peripheral blood cells recover. The forming rates and contents of CFU GM, BFU E and CFU Mix in the bone marrow and spleen 10 days postirradiation all increased more evidently and dose dependently in the treated mice. Endogenous spleen colony forming units on day 10 also increased with the dose of rhG CSF, becoming statistical signifficant in the 5.00 μg group as compared with the control. These results demonstrate that rhG CSF could not only promote the hemopoietic recovery of the granulocyte lineage but also accellerate the production of RBC and platelet; so, rhG CSF might be a potential medicine of first choice for treating acute radiation sickness.