呼吸窘迫综合征机械通气早产儿肺泡巨噬细胞表面mCD_(14)的表达

Expression of mCD_(14) on Alveolar Macrophage of Neonates with Respiratory Distress Syndrome Treated by Me-chanicalventilation

目的探讨肺泡巨噬细胞(AM)表面mCD14的表达在新生儿呼吸窘迫综合征(NRDS)发病和并肺炎中的作用。方法用流式细胞仪检测早产新生儿呼吸道清洗液中AM表面mCD14的阳性表达率.用酶联免疫吸附试验检测早产新生儿呼吸道清洗液中IL-1β、IL-8含量。结果1.病例组AM的mCD14阳性表达率[(54.772±17.341)%]高于对照组[(14.023±10.713)%],差异有显著性(t=-7.739P<0.001);病例组IL-1β含量[(263.220±69.015)ng/L]高于对照组[(73.979±40.850)ng/L].差异有显著性(t=-9.139P<0.001);病例组IL-8含量[(377.564±165.867)ng/L]高于对照组[(61.064±39894)ng/L],差异有显著性(t=-7,185P<0.001)。2.IL-1β和IL-8均与AM表面的mCD14阳性表达率呈显著正相关(r=0872,0847P均<0.01);IL-8与IL-1β含量呈显著正相关(r=0.861P<0.01)。结论mCD14在AM表面的高表达以及细胞因子IL-1β和IL-8在NRDS发病及并肺部炎症的病理生理过程中起重要作用。

Objective To investigate the mechanism of mCD14 expression on AM in the pathogenes of neonatal respiratory distress syndromes（NRDS）. Methods The expression of mCD14 on AM was analyzed with flow cytometry. Enzyme - linked immunosorbent assay was performed for detecting the concentration of IL- 1β and IL- 8. Results The percentage of mCD14 positive AM in experimental group [（54.772±17 .341）%] was higher than that in control group [（14.023±10.713）%]（t = -7.739 P〈0.001）;the concentration of IL- 1 in experimental group[ （263. 220 ± 69. 015）ng/L] was higher than that in control group [ （73. 979 ± 40. 850） ng/L]（t =-9. 138 P〈0.001）;the concentration of IL-8 in experimental group[（377.564 ± 165.867）ng/L]was higher than that incontrol group[（61.064±39.894）ng/L]（t= -7.185 P〈0.001）. 2. The percentage of mCD14 positive AM closely correlated with the concentration of IL- 1 and IL-8 （r=0.872,0.8470 respectively P〈0.01）;the concentration of IL-8 also closely correlated with IL-1β（ r= 0. 861 P 〈 0.01 ）. Conclusion The higher expression of mCD14 on AM may play an important role in pathophysiology of NRDS and the occurrence of lung inflammation and injury.