T淋巴母细胞淋巴瘤石蜡包埋组织中HOX11L2基因的表达及其临床意义

HOX11L2 expression and its clinical significance in paraffin-embedded T-lymphoblastic lymphoma

目的探讨在石蜡包埋T淋巴母细胞淋巴瘤(T-lymphoblastic lymphoma,T-LBL)组织中检测目的基因HOX11L2表达的可行性及其意义.方法采用RT-PCR及实时荧光定量PCR(RQPCR)方法,对54例T-LBL石蜡包埋标本进行HOX11L2mRNA检测.结果54例中有7例(13.0%)检测到HOX11L2mRNA表达.被检出的7例均为儿童青少年,中位年龄9(5～15)岁,均有前纵隔占位病变,其中5例Ki67增值指数(PI)≥80%,2例病变累及中枢神经系统.HOX11L2表达组与未表达组在发病年龄、Ki67 PI及有无纵隔肿块的差异具有统计学意义(P值均＜0.05);与未表达组相比,HOX11L2表达组的生存期较短(中位生存时间分别为11.5和7.0个月),两组差异具有统计学意义(P＜0.05);两组性别、初诊时临床分期、中枢神经系统以及骨髓受累与否差异无统计学意义.结论应用RT-PCR或RQ-PCR方法在石蜡包埋组织中检测目的基因的表达是可行的.HOX11L2mRNA表达高发于儿童,可能与部分儿童T-LBL的发生以及预后不良有关.

Objective To determine the feasibility of detecting target gene expression and evaluate the expression of homeobox gene HOX11 L2 as well as its clinical significance in paraffin-embedded T-lymphoblastic lymphoma T-LBL. Methods HOX11 L2 mRNA was anatvzed by RT-PCR and real-time fluorescent quantitative PCR （RQ-PCR） on paraffin-embedded tissues in 54 cases of T-LBL. Results HOX11 L2 expression was observed in 7 cases （ 13.0% ）. The age ranged from 5 to 15 years, with a median of 9 years. All of the HOX11 L2 expressed patients had prior mediastinal masses, and 5 of them expressed high level of Ki67 antigen （ PI≥80% ）. Symptoms of CNS invasion were diagnosed in 2 cases. Significant differences in the age ratio （P 〈0.05 ） , the level of Ki67 antigen （P〈 0.05 ） and the incidence of mediastinal involvement （ P 〈 0.05 ） were observed between patients positive or negative for HOX11 L2. Patients positive for HOX11 L2 had a worse outcome than those negative for HOX11 L2, with median survival of 7 and 11.5 month, respeetively. No statistic difference was found in sex, clinical stages, CNS and bone marrow involvement between HOX11 L2 positive and negative groups. Conclusions Detection of target gene expression in paraffin-embedded tissues is feasible by RT-PCR or RQ-PCR. HOX11 L2 expression was limited in childhood with T-LBL and probably associated with the development and poor prognosis of some pediatric T-LBL patients.