摘要
为获得柞蚕核型多角体病毒(Antheraea pernyinucleopolyhedrovirus,ApNPV)基因组序列,采用随机克隆方法,建立ApNPV的质粒基因文库,并通过对插入片段进行克隆鉴定和序列分析,获得了编码包涵体衍生型病毒特异囊膜蛋白基因odv-e56。该基因上游具有晚期调控保守序列TTAAG,是一个晚期表达基因,阅读框为1125bp,共编码374个氨基酸。核苷酸和氨基酸同源性比较结果表明:ApNPV的odv-e56基因与黄杉毒蛾多角体病毒(OpNPV)和云杉卷叶蛾核型多角体病毒(CfNPV)的同源性较高,而与松树小叶蜂核型多角体病毒(NlNPV)和松环螺旋多角体病毒(NsNPV)的同源性最低。由此认为,杆状病毒科的odv-e56基因在进化上存在两种进化方式:一类以点突变为主,基因长度变化不明显;另一类突变以小片段的碱基增减为特征。
In order to obtain the whole genome of Antheraea pernyi nucleopolyhedrovirus （ApNPV）, we established the genomic library by digesting the genome DNA with BamH Ⅰ and Sal Ⅰ and cloning with pGEM3Z. Results of measuring and megaligning the inserted sequence showed that the odv-e 56 gene which coded the occlusion-derived virus envelope fusion protein is acquired. It has a late gene motif TTAAG and behaves similary to all baculovirus late genes, its open reading frame （ORF） has 1 125 base pairs and encodes 374 amino acids. By comparing the identities of nucleotide acid sequences and amino acid sequences with homologous baculoviruses, we found that the ApNPV gene odv-e 56 was high homologous with that of Choristoneura fumiferana nucleopolyhedrovirus （CfNPV） and Orgyia pseudotsugata nucleopolyhedrovirus （ OpNPV）, but lower homologous with that of Neodiprion lecontei nucleopolyhedrovirus （NINPV） and Neodiprion sertifer nucleopolyhedrovirus（NsNPV）. From the evolution of the nucleopolyhedrovirus, we concluded that the evolution of ApNPV odv-e 56 had two kinds of ways. one is point mutation and the other is short of amino sequence.
出处
《蚕业科学》
CAS
CSCD
北大核心
2005年第3期300-305,共6页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金项目(编号30271007)。
