人肺腺癌细胞经化疗药物作用后对LAK细胞的敏感性

The Susceptibility of Human Pulmonary Adenocarcinoma Cell Treated with Chemotherapeutic Drugs to LAK Cells

在体外实验中研究了８种化疗药物作用后的个旧人肺腺癌细胞（ＧＬＣ－８２）对ＬＡＫ细胞的敏感性。ＧＬＣ－８２细胞本身对化疗药物不敏感，经药物作用２４小时后用３Ｈ－ＴｄＲ释放法检测ＬＡＫ细胞对其杀伤率。结果表明：顺铂（ＣＤＤＰ）、卡铂（Ｃａｒｂｏ）、阿霉素（ＡＤＭ）、丝裂霉素（ＭＭＣ）及长春新硷（ＶＣＲ）处理后的ＧＬＣ－８２细胞对ＬＡＫ细胞的敏感性增强；环磷酰胺（ＣＴＸ）、足叶乙甙（ＶＰ１６）及５－氟脲嘧啶（５－Ｆｕ）则无明显影响。实验提示：对化疗不敏感的肺腺癌用ＣＤＤＰ、Ｃａｒｂｏ、ＡＤＭ、ＭＭＣ及ＶＣＲ后加用ＬＡＫ细胞可提高肺癌治疗的有效率。

Human Gejiu pulmonary adenocarcinoma cell line(GLC-82) was found to be resistant to anticancer agents. This work studied the susceptibility of the GLC-82 treated with 8 kinds of anticancer agents to LAK cells in vitro using 3H-TdR release assay. It was shown that GLC-82 treated with cisplatin, carboplatin, adriamycin, mitomycin C and vincristine underwent lysis by LAK cells after short term culture, while with cyclophosphamide, etoposide and 5-fluorouracil did not.It suggests that lung cancer resistant to anticancer agents could be treated with LAk cells after administration of CDDP, Carbo, ADM, MMC or VCR.