小球藻的筛选和异养培养

Isolation and heterotrophic culture of Chlorella sp

从北京清河筛选出了具有异养能力的一株藻类,经初步鉴定为小球藻.在异养批量培养条件下,研究了不同氮源和碳氮比对筛选小球藻生长的效应.当葡萄糖作为惟一碳源时,硝酸钾和尿素都可以分别作为惟一氮源支持小球藻快速持续生长,而氯化铵作为惟一氮源时因使培养物中的pH快速降低而抑制了小球藻的进一步生长.当葡萄糖和硝酸钾分别作为小球藻生长的惟一碳源和氮源时,在碳氮质量比从5到20范围内,小球藻的生长随碳氮质量比的升高而明显增加,最大OD680nm达到了73.8.

A strain of microalga that has a strong ability in the utilization of organic compounds was isolated from Qinghe River in Beijing and identified as Chlorella sp. The effects of nitrogen compounds and carbon-to-nitrogen mass ratio on the growth of this isolated Chlorella sp were investigated in batch cultures. Using glucose as the sole carbon source, both potassium nitrate and urea could be used as the sole nitrogen sources to support the continuous growth of Chlorella sp, respectively. When ammonium chloride was used as the sole nitrogen source, the growth of Chlorella sp could be apparemly inhibited by the rapid decrease in pH of culture solution. When glucose and potassium nitrate were used as the sole carbon and nitrogen sources, respectively, the growth ofChlorella sp increased with the increase of carbon-to-nitrogen mass ratio in range from 5 to 20, and the maximum OD680nm of 73.8 was obtained.