肺结核患者血清瘦素和肿瘤坏死因子-α检测及其相关性

Detection of serum leptin,TNF-α and their correlation analysis in patients with lung tuberculosis

目的检测肺结核患者血清瘦素(leptin)和肿瘤坏死因子-α(TNF-α)浓度并分析其相关性。方法采用放射免疫法测定48例活动性肺结核患者和30例正常人的血清瘦素和TNF-α。同时测定两组实验者的细胞免疫功能,分析瘦素与这些参数的相关性。结果肺结核患者的血清瘦素[(3.0±1.74)μg/L]水平显著高于正常对照组[(1.32±0.18)μg/L(P<0.01)]。而TNF-α血清浓度[(1.8±0.84)μg/L]也高于对照组[(0.79±0.26)μg/L](P<0.01),两组间无显著相关性。肺结核患者的T细胞亚群CD3+T、CD4+T、CD8+T和总淋巴细胞计数显著下降,且与血清瘦素呈负相关关系。结论肺结核患者的血清高瘦素水平可能参与了细胞免疫功能的降低。

Objective To detect concentration of serum leptin and tumor necrosis factor-α（TNF-α） in patients with active lung tuberculosis and to analysis their correlation. Methods Serum leptin and TNF-α concentrations were measured by radioimmunossay in 48 patients with lung tuberculosis and in 30 controls. Cell mediated immunity was also measured,the correlation between leptin and other parameters was analyzed. Results Serum leptin concentration in 48 patients with lung tuberculosis was higher significantly than that in control, serum TNF-a concentration was higher than in control, too. There was no correlation between TNF-a and leptin. Compared with control,T-lymphocyte subsets and total lymphocyte count decreased obviously, and serum leptin level was negatively correlated with the parameters for cell-mediatecl immunity. Conclusion The higher concentration of serum leptin in patients with lung tuberculosis perhaps involved in the decrease of T cell-mediated immunity.