膀胱癌中Ha－ras癌基因点突变和ras　P21表达的研究

Detection of Point Mutation of Ha-ras Oncogene and ras P21 Expression in Bladder Cancer

应用聚合酶链反应（ＰＣＲ）技术和寡聚核甘酸探针杂交法检测膀胱癌中Ｈａ－ｒａｓ癌基因第１２位密码子Ｇ→Ｔ突变，同时应用免疫组织化学方法检测了ｒａｓＰＺＩ在膀胱癌中的表达。在２９例膀胱癌中有１０例存在Ｈａ－ｒａｓ癌基因第１２位密码子点突变，突变率为３４．５％，有１８例ｒａｓＰ２１表达为阳性，阳性率为６２．１％。结果提示Ｈａ－ｒａｓ癌基因第１２位密码子点突变和ｒａｓＰ２１的表达与膀胱癌病理分级、临床分期和膀胱癌的复发密切相关。Ｈａ－ｒａｓ癌基因第１２位密码子点突变与ｒａｓＰ２１的表达是一致的。

G→T mutation on codon 12 of Ha-ras oncogene was detected in bladder cancer by means of polymerase chain reaction (PCR) technics and blothy bridization assays with specific oligonucleotide probe. The expression of ras P21 was investigated by immunohistochemical techniec.In 10 of 29 bladder cancer specimens point mutation in codon 12 of Ha-ras oncogene was found,with a mutation rate of 34.5%.The P21 was positive in 18,with a positive rate of 62. 1%.The results showed that there was relationship between the point mutation of Ha-ras oncogene and ras P21 expression and the pathological grade,clinical stages and recurrence of tumor.The expression of ras P21 was consistent with point mutation in codon 12 of Ha-ras oncogene.