骨髓间充质干细胞移植治疗Duchenne肌营养不良型mdx小鼠后的肌电图和dystrophin蛋白表达改变

Electromyogram and dystrophin expression in mdx mice with mesenchymal stem cells transplantation

目的研究骨髓间充质干细胞(MSCs)移植治疗Duchenne肌营养不良(DMD)模型鼠mdx鼠后肌电图改变及dystrophin蛋白表达变化。方法分离培养正常小鼠MSCs局部肌肉注射移植入DMD模型鼠mdx鼠,数周后观察肌电图改变及dystrophin蛋白表达变化。结果MSCs移植后mdx鼠肌电图明显改善,dystrophin蛋白阳性表达肌纤维增加明显。结论MSCs局部骨骼肌肌肉内细胞移植治疗mdx鼠有一定效果。

Objective To investigate the electromyogram and dystrophin expression as influenced by mesenchymal stem cells implantation into skeletal muscle in mdx mice（ Duchenne muscular dystrophy model）. Methods MSCs isolated from mice bilateral tibias were cultured and injected into the gastrocnemius muscle of mdx mice and normal mice, 16 weeks after implantation, the MUAP voltage and dystrophin protein expressing was analysed. Results 16 weeks after implantation, MUAP voltage and dystrophin protein expressing improved significantly. Conclusion MSCs transplantation can be achieved by injecting MSCs into muscle and treat mdx mice.