丙型肝炎高变区1合成肽体外刺激HCV感染PBMC细胞因子分泌的研究

Cytokines secretion by peripheral blood mononuclear cells from hepatitis C patients after stimulation with synthetic peptides at the highly variable region

目的检测丙型肝炎病毒(HCV)高变区1(HVR1)模拟表位刺激自然感染患者外周血淋巴细胞后的细胞因子水平变化。方法细胞因子检测运用ELISA、流式细胞仪(FACS)淋巴细胞分类以及计算机模拟多肽抗原性。结果(1)1号和5号合成肽刺激HCV患者外周血单核细胞(PBMC)后,患者PBMC出现了明显的增殖;(2)培养上清中干扰素γ(IFNγ)、白介素4(IL-4)、白介素10(IL-10)都有不同程度的提高,白介素2(IL-2)和肿瘤坏死因子α(TNF-α)的水平并没有提高;(3)增殖细胞主要为CD4阳性淋巴细胞。结论我们设计的HCV高变区1合成肽引发的免疫因子的释放倾向于TH2类因子。

Objective Test the cytokines secretion by peripheral blood mononuclear cells （PBMC） from hepatitis C patients after stimulation with highly variable region （ HVR1 ） synthetic peptides. Methods ELISA was used to test the cytokines secretion, flow cytometry to group the proliferated cells, and the antigencity of synthetic peptide was predicted by the computer modeling. Results （ 1 ） There were PBMC proliferation when stimulated by HVR1 antigen synthetic peptides. （2） There was a rise of IFN-γ, IL-4 and IL-10. But no rise of IL-2 and TNF-γ, was found. （3） The proliferated cells were mainly CD4 ^＋ lymphocytes. Conclusion PMBC from hepatitis C patients tend to secret Th2 cytokines after stimulation with HVR1 designed by the authors.