甲醛对雄性小鼠生殖细胞毒作用的研究

Study on formaldehyde's effect on germ cells in Male Mice

研究了液态和气态甲醛对雄性小鼠生殖细胞的毒作用.选用SPF级昆明种纯系雄性小鼠作为研究对象,液态甲醛染毒剂量分别为0.20,2.00,20.00 m g/kg体重,采用腹腔注射染毒7 d,观察睾丸组织的病理改变,并计算脏器系数、精子存活率、活动率、精子数及畸形率.气态甲醛染毒剂量分别为0.5,1.0,3.0 m g/m3,连续动态染毒72 h,于首次染毒后第15天观察睾丸细胞的微核率.结果表明,液态甲醛能引起睾丸组织病理损伤,脏器系数、精子存活率、活动率和精子数减少,畸形率升高;气态甲醛能诱导早期精细胞微核率增加,均呈现一定的剂量-反应关系.液态甲醛和气体甲醛均具有小鼠生殖细胞毒性.

In order to explore the effects of liquid and gaseous formaldehyde on germ cells in male mice, methods SPF-class Kun-Ming male mice were used in experiment, the liquid formaldehyde expose concentration was 0.20, 2.00, 20.00 mg/kg, bw, the agent was i.p. administrated everyday for 7 days. The pathological change was observed and the spermatids weight coefficient and viability, activity, number of sperms and the sperm deformity rate were measured. The gaseous formaldehyde expose level was 0.5 mg/m^3, 1.0 mg/m^3, 3. 0 mg/m^3, the agent was administrated by continuous dynamic inhalation. After first expose for 15 days, the micronuclei rate was measured. The results were. the spermatid pathological damaged, tissue coefficient, viability, activity, number of sperm decreased, and the sperm deformity increased; Gaseous formaldehyde expose could induce the micronuclei of early spermatids. Both expose methods showed a dose-dependent relationship. The conclusion was liquid and gaseous formaldehyde had sperm toxicity in mice.