原发性高血压患者动态脉压与细胞因子的相关性

Relationship between cytokine and ambulatory pulse pressure in patients with essential hypertension

目的:探讨原发性高血压患者动态脉压与细胞因子的相关性。方法:经动态血压监测将122例原发性高血压患者按24 h平均脉压(24 h PP)分为3个组:24 h PP≤60 mmHg(1 mmHg=0.133 kPa)组,32例;24 h PP>60-75 mmHg组,48例;24 h PP>75 mmHg组,42例。采用双抗体夹心酶联免疫吸附法(ELISA)分别测定血清白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)、可溶性IL-2受体(sIL-2R)水平。结果: ①3个亚组IL-6、TNF-α、IFN-γ、sIL-2R差异有统计学意义(P<0.05);随24 h PP增加,IL-6、TNF-α、sIL-2R逐渐升高,IFN-γ逐渐降低。②24 h PP、白昼(d)平均脉压(dPP)、夜间(n)平均脉压(nPP)与IL-6、TNF-α、sIL-2R正相关(P<0.01),与IFN-γ负相关(P<0.01),且均以nPP相关性最好。多元线性回归分析显示,IL-6、TNF-α、sIL-2R与24 h PP、nPP直线相关(24 h PP:R=0.413,F=7.012,P<0.01;nPP:R=0.502,F=7.425,P< 0.01)。结论:高动态脉压的原发性高血压患者存在细胞因子的变化,细胞因子可能参与高动态脉压形成和靶器官损害。

Objective：To study the relationship between serum levels of cytokine and ambulatory pulse pressure （AMPP） in patients with essential hypertension. Method：One hundred and twenty-two patients with essential hypertension were divided into three subgroups according to their 24 hours mean pulse pressure（24 h PP） scale （24 hPP≤60 mmHg, n =32; 60〈24 hPP≤75 mmHg , n =48; 24 hPP〉75 mmHg, n=42） . The levels of interleu- kin-6 （IL-6）, tumor necrosis factor-α（TNF-α）, interferon-γ（IFN-γ） , soluable interleukin-2 receptor （ sIL - 2R） in serum were measured by enzyme linked immunosorbent assay （ELISA） . Relust：①The serum levels of IL-6, TNF-α, IFN-γ, sIL-2R were significantly different among the three subgroups （all P〈0.05 ） . IL-6, TNF-α, slL-2R rose progressively with 24 hPP, while IFN-γ, decreased progressively with 24 hPP. ②There were significantly positive correlation between 24 hour , day-time , night-time pulse pressure （24 hPP, dPP , nPP） and IL-6, TNF-α, sIL-2R（ P〈0.01） , especially nPP; and negative correlation between 24 hPP, dPP , nPP and IFN-γ（ P〈0.01） , especially nPP; On multiple regression analysis , IL-6, TNF-α, IFN-γ and sIL-2R were deeply related to 24hPP and nPP （24hPP： R=0.413,F=7.012,P〈0.01;nPP：R=0.502,F=7.425,P〈0.01）. Conclusion： The levels of cytokine in patients with high AMPP were changed , cytokine might play a role in high AMPP and target organs damage in patients with essential hypertension.