瘦素对人脐静脉内皮细胞血管内皮生长因子表达的影响

Effect of leptin on VEGF expression in human umbilical vein endothelial cells

目的:探讨瘦素对人脐静脉内皮细胞(HUVECs)血管内皮生长因子(VEGF)表达的影响。方法:用不同浓度的瘦素刺激原代培养的HUVECs,检测HUVECs表达VEGF的情况。结果:在相同作用时间下,随着瘦素浓度的升高,VEGF蛋白及VEGF mRNA的表达也随之升高,经统计学检验有相关性;在相同瘦素浓度下, 随着作用时间的延长,VEGF蛋白及VEGFmRNA的表达也随之升高,且有相关性。结论:瘦素可以刺激HU- VECs表达VEGF而且呈时间和剂量相关性。

Objective： To investigate the effect of leptin on the expression of VEGF in cultured human umbilical vein endothelial cells （HUVECs）. Method：The leptins in different concentrations were used to stimulate the primary cultured HUVECs and then detected the level that the HUVECs express the VEGF. Result：With the same time, the expression of VEGF protein and VEGF mRNA increased when the level of leptin increased; meanwhile, with the same concentration of leptin, the expression of VEGF protein and VEGF mRNA increased when the time extended. Both are significant when they are analyzed by the statistics. Conclusion： The expression of VEGF is concerned with the level of leptin and the time that the leptin acts on the HUVECs.