凡纳滨对虾类Ig定性和定位的初步研究

On immunolocalization of Ig-like proteins in shrimp Litopenaeus vannamei

以凡纳滨对虾(Litopenaeus vannamei)为研究对象,运用Western-blotting和免疫组织化学等现代免疫学方法,对对虾中的类Ig(immunoglobulin,免疫球蛋白)蛋白进行定性和定位研究。结果发现,虾血清中有两种分子质量约为70 ku和62 ku的蛋白可与抗人Ig发生特异性的结合。同时,虾血细胞——无颗粒细胞的外膜及虾类淋巴器、肝胰脏、心脏、胃和肠等5种组织器官的石蜡切片与抗人Ig反应呈不同程度的阳性,其中类淋巴器和肝胰脏显色较深。提示,虾体内确实存在能与抗人Ig发生特异性结合的类Ig蛋白,其不仅存在于虾血清中,还存在于无颗粒细胞、类淋巴器和肝胰脏等组织中,为对虾的免疫系统的研究和疾病的防治奠定基础。

In the paper, an attempt was made to analysis the immunolocalization of Ig-like proteins that bound to anti-human Ig （immunoglobulin, Ig） in shrimp L. vannarnei using modern methods of Western-blotting and immunohistochemistry. The results showed two types of protein which reacted strongly with goat anti-humanlg in the shrimp hemolymph, whose molecular weights were about 70 ku and 62 ku respectively. And the outer membrane of the hyalocyte and five tissues including lymphatic-like organ, hepatopancreas, heart, intestine and stomach were also bound with anti-human Ig specially, of which, the lymphatic-like organ and hepatopancreas were stained stronger than other samples. These results indicated that there do exist Ig-like proteins in the shrimp, which localized not only in hemolymph but also in haemocytes, lymphatic-like organ and hepatopancreas.