实验用猪胰腺和小肠组织总RNA提取及鉴定

Extraction and appraisement of total RNA of porcine pancreas and small intestine

目的:探讨提取猪胰腺和小肠组织总RNA并尽量减少RNA降解的方法,为胰腺和小肠组织高质量RNA的提取和进一步研究奠定基础。方法:取新鲜猪胰腺和小肠组织,RNA提取采用酸性一步法。结果:RNA琼脂糖凝胶电泳显示RNA没有明显降解。结论:胰腺和小肠组织RNA酶含量丰富,极易降解RNA。操作速度、RNA提取试剂盒的质量和防止RNA污染的综合措施是成功制备高质量RNA的关键。

Objective： To investigate approaches of extracting total RNA of porcine pancreas and small intestine and at the same time minimizing the denaturation of RNA, and to pave the way for the improved extraction of RNA of pancreas and small intestine later on and further research. Methods ：Fresh porcine pancreas and small intestine were used. Acid one step method was applied to extract RNA. Results： Agarose electrophoresis shows there is no obvious denaturation of RNA. Conclusion. Rnase is rich in pancreas and small intestine and may easily denature RNA. The keys in extracting RNA of highquality are the speed of operation, the quality of RNA extraction kit and effective ways to defend RNA from pollution.