早世代稳定水稻的ISSR标记

Study on Early Stability by ISSR Markers in Rice

2个早世代稳定亲本与4个普通水稻杂交,F2代出现了8个早世代稳定株系。遗传分析表明,稳定株系出现时杂交F1群体分离,同一组合的F2群体中既出现农艺性状整齐一致的稳定株系,又出现按孟德尔分离的株系。利用26个ISSR引物对F2稳定株系进行分子标记,结果表明稳定株系出现4种类型的标记带型:母本带型出现,父本带消失;父本带型出现,母本带消失;由双亲的部分标记带组合而成;双亲标记带型消失,而出现新的标记带型。没有出现由双亲标记带组合而成标记带型。ISSR引物900标记的2000bp标记带可以将稳定株系与普通水稻材料划分为两类群。

In the F2 population crossed from two early stability rice （ Oryza sativa L. ） with four cultivars, eight uniform strains were recorded. Genetic analysis showed that both uniform strains with uniform agronomic characteristics and segregated strains segregating in Mendelian manner were observed in F2 population of the same combination. Four types of marker-bands were obtained after the F2 uniform strains were marked by 26 ISSR primers：type Ⅰ , maternal marker bands present, and paternal marker bands absent; type Ⅱ, paternal marker bands present,and maternal marker bands absent;type Ⅲ , parts of maternal and paternal marker bands present,and the others absent; type Ⅳ, new recombined marker bands appeared, and all maternal and paternal marker bands absent. But segregating strains showed heterzygosity marker bands, maternal and paternal marker bands being present. The uniform strains and normal strains in rice might be grouped into two classes on the basis of the 2 000 bp marker band amplified by the ISSR marker primers No. 900. This result would provide experimental basis for the study on genetic mechanism of early stability in rice.