异羟肟酸比色法测定D-核糖发酵液中葡萄糖酸的含量

Determination of Gluconate Concentration in Broth of D-ribose Fermentation Using the Hydroxamate Method

对异羟肟酸比色法测定葡萄糖酸含量的方法进行了改进。通过考察不同葡萄糖浓度对异羟肟酸比色法测定葡萄糖酸的影响,得出葡萄糖浓度对葡萄糖酸测定影响的干扰方程,据此计算得到的葡萄糖酸量可以排除葡萄糖的影响,使得检测结果更为准确。方法适用于D-核糖发酵液中葡萄糖酸的测定。

Glucose interferes with gluconate measurement using the hydroxamate colorimetric method. This study focused on the effect of glucose on the measurement of gluconate. Through measuring a series of solutions containing different amount of glucose and gluconate, a formula for calculating gluconate concentration was obtained, with which the interference caused by glucose could be eliminated. This method was applied to determination of gluconate concentration profile during D- ribose fermentation.