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高效液相色谱法检测玉米中的伏马毒素B_1和B_2 被引量：17

Liquid Chromatographic Determination of Fumonisin B_1 and B_2 in Corn
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摘要 采用柱前衍生,高效液相色谱分离,荧光分光光度法对玉米中伏马毒素B1和B2进行了定量测定,其线性范围分别为1.04～16.67ng(FB1)和1.67～26.67ng(FB2),检测限分别为0.037和0.028mg/kg。以该方法对加标玉米中的伏马毒素进行回收率测定,加标水平为0.1～2mg/kg时,FB1和FB2的平均回收率分别为80.1%和82.0%。 A high-performance liquid chromatographic method was developed for the quantitative determination of fumonisins B1 （FB1） and B2 （FB2） utilizing pre-column derivation and fluorescence detection. Calibration curves of FB1 and FB2 were in the range of 1.04-16.67ng（FB1）and 1. 67-26.67 ng （FB2） respectively, the limits of detection were approximately 0.037（FB1） and 0. 028mg/kg（FB2）. The average recoveries of FB1 and FB2 were found to be 80.1% and 82.0%.
作者 权英 王硕 韩英素 张燕 王俊平
机构地区 天津市"食品营养与安全"重点实验室
出处 《食品与发酵工业》 CAS CSCD 北大核心 2005年第8期87-90,共4页 Food and Fermentation Industries
基金 天津科技大学重点基金资助项目
关键词 伏马毒素 高效液相色谱 玉米 高效液相色谱法 检测限 荧光分光光度法 平均回收率 液相色谱分离 定量测定 柱前衍生 fumonisins, HPLC, corn
分类号 TS210.7 [轻工技术与工程—粮食、油脂及植物蛋白工程]
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参考文献16

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  • 2Ross P F, Rice L G, Osweiler G D, et al. A review and update of animal toxicoses associated with fumonisin-contaminated feeds and production of fumonisins by Fusarium isolates[J]. Mycopathologia, 1992, 117:109-114.
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  • 4Ildiko' Barna-Vetro', Erzse'bet Szabo', Be'la Fazekas, et al.Development of a sensitive ELISA for the determination of fumonisin B1 in cereals[J ]. J Agric Food Chem, 2000, 48:2 821-2 825.
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  • 6U S Food and Drug Administration. Draft guidance for industry: fumonisin levels in human foods and animal feeds; availability. Fed. Regist. 65, 35945. http://www, cfsan, fda.gov/-dms/fumongui, html (accessed 20 June 2002).
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  • 10Chen J, Mirocha C J et al. Xie Wet al. Production of the mycotoxin fumonisin B1 by Alternaria alternata f. sp. lycopersici[J]. Appl Environ Microbiol, 1992, 58. 3 928-3 931.

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共引文献38

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引证文献17

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食品与发酵工业
2005年 第8期

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