不同搅拌速度对松口蘑菌丝体发酵的影响

Study on Submerged Culture of Tricholoma Matsutake

在摇瓶发酵条件优化的基础上,研究了松口蘑在25L搅拌式发酵罐上的分批放大实验,探讨了不同搅拌转速对菌丝生物量、生产强度、pH、溶氧及对菌丝形态的影响,在发酵温度25℃,通风量0.8vvm,罐压0.5kg/cm2的条件下,最佳搅拌转速为100r/min。发酵培养8d后,发酵液由浑浊变为澄清,上清液亮黄色,菌丝形态呈均匀颗粒状,菌丝生物量增加趋于平缓,最高可达11.5g/L。

On the basis of flask fermentation of Tricholoma Matsutake, it was cultured by 25 L-fermentor. The biomass of mycelium, dissolved oxygen, pH and the shape of mycelium were studied on the influence of different rotors. The experimental results showed that the best rotating speed was 100 r/min when the temperature was 25℃ , the volume ratio of flow air was 0.8vvm and the pressure was 0.5 kg/cm^2. After 8 days, the broth was transparent and yellow, the mycelium was round pellet, the biomass of mycelium reached the highest lever which was 11.5g/l.