玉米同核异质不育系小孢子发育的mRNA多态性分析

Polymorphism Detection and Analysis of mRNA from Isonucleur and Allocytoplasm Maize Male Sterility by cDNA-RAPD

应用cDNA-RAPD技术分析了玉米同核异质不育材料在小孢子发育过程中花粉发育相关基因表达的差异。结果表明,在小孢子发育的相同时期,引物S330在48-2背景下的不同胞质之间存在差异,不同时期差异表现不一;引物S330在黄早四背景下的不同胞质之间未检测到差异,并且小孢子发育的3个时期表现一致。对同核异质不育系和同质异核不育系的比较发现,核背景对mRNA转录的影响大于细胞质,不育胞质T、C、S在48-2背景和黄早四背景下的差异表现不一。根据S330在48-2背景下的扩增结果,发现基于mRNA多态性的相似性比较结果与不育细胞质的败育特点及败育时期具有较好的一致性,即配子体不育类型与保持系的相似性高于孢子体不育类型,孢子体不育类型之间的相似性高于孢子体不育与配子体不育的相似性。

cDNA-RAPD was employed to compare to the gene expression in developing anther among 2 sets of isonucleur and allocytoplasm maize male sterility.The results indicated that at the same development stage of microspore, primer S330 produces different bands under 48-2nucleur background,but no difference was detected among different cytoplasms at 3 development stages of microspore under the Huangzaosi nucleur background. The effeet of nueleur background on the mRNA polymorphism was bigger than the effect of cytoplasms. Based on the similarity analysis of mRMA polymorphisms, S48-2has higher similarity with N48-2 than T48-2 and C48-2, among 3 different male sterile cytoplasms, T48-2 and C48-2 has higher similarity than another. These results were identical to the abortion style and characteristic of gametophyte male sterility and sporophyte male sterility.