东北春玉米骨干自交系组织培养再生系统研究

Study on Regeneration of Spring Maize Inbred Lines in Northeast of China

东北玉米骨干自交系在诱导愈伤组织和植株再生中,基因型差异非常显著。自交系7922最高愈伤诱导率达89.1%,芽苗率为66.4%;而最低的921诱导率仅有18.9%,芽苗率为0。再生途径主要有两种:7922主要以体胚发生途径为主,4122、8902主要以器官发生途径为主。2熏4-D是体胚诱导不可缺少的植物生长激素,适当的细胞分裂素能促进诱导4112、8902不定芽的产生。而且,再生途径并不是一成不变的,在一定条件下可以相互转变。

There existed great differences among maize genotypes when immature embryos were cultured for plant regeneration. 7922 showed the highest frequencies of callus induction and shoot formation which were 89.1%, and 66.4% respectively. While the lowest was found in 921, the induction and regeneration frequencies were 18.9% and 0 respectively. There are two regeneration pathways. 7922 tended to regenerate via somatic embryogenesis, 4112, 8902 are likely to regenerate via organogenesis. 2,4-D was essential for inducing somatic embryoids, and suitable cytokin can promote adventitious shoot formation of 4112 and 8902. Furthermore, the regeneration pathways are interchangeable under different culture conditions.