玉米瘤黑粉病抗性鉴定技术研究

Inoculation Technique for Identification of Corn Resistance to Common Smut

比较了不同接种方法、接种时期、接种量及接种液预处理方法对大田玉米瘤黑粉病发病率的影响,结果表明:大田玉米瘤黑粉病的最佳接种时期为6叶1心期,最佳接种方法为孢子液注射法和菌沙法,注射法最适宜接种孢子浓度为7～8×103个/mL,孢子液预处理能明显提高接种效果。

Effect on incidence rate of common smut, Ustilago maydis, in field conditions by different inoculating methods, inoculating time, dosages for inoculation and the pretreatment methods of the teliospore suspension before inoculation were compared. The results showed that the optimal time of inoculation is at 6 leaf-stage of corn plant （V4 stage）, two most effective inoculating methods are to inject the teliospore or put the mixture of teliospore and fine sand into the whorl of the corn plants, which caused the highest incidence rate of common smut. The optimal dosage of the pathogen for injection is at 7 - 8×10^3 telispore/mL. The pretreatment for teliospore suspension before inoculation significantly increased the smut incidence.