不同穗发芽抗性的小麦胚对ABA敏感性的蛋白质组分析

Proteome Analysis on ABA Sensitivity of Wheat Embryo Isolated from Cultivars Resistant or Susceptible to Sprouting

小麦穗发芽抗性与其对ABA敏感性关系密切,一般认为ABA处理可导致基因表达的变化。本研究用不同穗发芽抗性的白皮小麦品种陕优225和冀麦1号扬花后25d的胚在±1μmol·L-1ABA溶液中处理48h后进行蛋白质组分析。依据蛋白质表达差异,鉴定出胚发育后期丰度蛋白(LEA)、NAD(P)H脱氢酶亚基Ⅰ、盐胁迫后根表达的蛋白(RS1)、生长素反应蛋白IAA19、20kD的钙结合蛋白、钙调蛋白(CAM)和肌动蛋白解聚因(ADF5)等7个蛋白点。按其表达丰度大致可分为两类:第一类如LEA,在抗穗发芽品种中的表达量比易穗发芽品种高,ABA处理可以增加其表达。第二类如生长素反应蛋白IAA19,在易穗发芽品种中的表达量较高,ABA处理可以降低其表达。抗穗发芽品种的离体胚更易于表达ABA上调的蛋白质。这些结果为了解抗穗发芽基因表达蛋白质的过程提供了实验数据。

There is a significant relationship between resistance to sprouting and ABA sensitivity in wheat. Variance of protein expression will take place after ABA treatment. In this study, wheat embryos isolated from resistant cultivar Shanyou 225 and susceptible cultivar Jimai 1 at 25th day after anthesis were incubated with or without ABA solution to identify ABA sensitivity, and then protein expression in the embryos was studied by proteome analysis. The results indicated that seven protein spots could be recognized according to the level of expression. They are NAD（P）H dehydrogenase, late embryogenesis abundant （LEA） protein Dc3, actin-depolymerizing factor 5 （ADF-5）, calmodulin-related protein, salt-stress root protein RS1, 20 kD calcium-binding protein and auxin-responsive protein IAA19. These proteins could be divided into two categories. The abundance of some proteins, such as LEA, in dormant embryo was higher than that of non-dormant embryo, the expression level of these proteins are up-regulated by ABA. On the other hand, the auxin-responsive protein IAA19 with higher abundance in non-dormant embryo than dormant embryo was identified, its expression level was down-regulated by ABA. The proteins up-regulated by ABA are prone to express in water than ABA treatment in embryos of sprouting resistant genotype. These data are useful to study protein expression process of sprouting resistant genes.