牛肌腱胶原蛋白的提取及多孔支架的制作

Collagen Extraction from Bovine Tendon and Porous Scaffold Manufacture

目的:从牛肌腱中提取I型胶原,制作适宜于人工骨材料的基质支架。方法:酸溶法提取牛肌腱中的胶原蛋白,并经盐析、透析提纯,用紫外分光光度法、氨基酸分析、常规组织切片、扫描电镜对提纯的胶原进行检测。冷冻干燥法获得多孔胶原基质支架。结果:提纯精制的胶原蛋白符合I型胶原蛋白的特征。胶原支架块具有疏松多孔的三维结构,孔隙率90.0%以上。结论:提取的胶原符合I型胶原的特点,适宜于制作成为人工骨生物材料的基质支架。

Type Ⅰ collagen was extracted and purified from bovine tendon, then made to base matrix scaffold of artificail bone. Methods： Raw collagen was extracted from bovine tendon in acetic acid, then by salting out, dialysis and lyophilization , pure collagen scaffold was acquired. Ultraviolet spectrophotography , amino acid analysis ,histology observtion , scanning electron microscope were used to evaluate the pure collagen. Results： The purified collagen was coincidence with type Ⅰ collagen. Collagen matrix scaffold was similar to sponge , the ratio of porosity was above 90%. Conclusion：Collagen matrix scaffold had suitable strcture to form base scaf- fold of artificial bone.