用基因芯片研究不同皮肤组织创面愈合过程中同源异形框基因的差异表达

Differential display of homeobox gene expressions in the normal, wounded human fetal and adult skins by DNA microarray

目的探讨人胎儿及成人皮肤创面愈合过程中同源异形框基因的差异表达。方法采用包含有14000个靶基因cDNA克隆的基因芯片技术,对正常成人和胎儿及其创面愈合过程中同源异形框基因族的表达进行对比观察。结果正常成人和胎儿及其创面愈合过程中同源异形框基因族呈现差异表达,其中以PRX2、HOXB13、HOXB6和HOXB7的差异表达为显著。结论同源异形框基因的差异表达表明正常成人和胎儿皮肤的发育生物学状态存在差异,可能是胎儿和成人皮肤不同创面愈合方式的根本原因。

Objective To explore the differential expression of homeobox genes in the normal , wounded human fetal and aduh skins and its significance in fetal scarless healing. Methods Gene chips containing 14 000 human genes were used to investigate homeobox gene expressions of the normal, wounded human fetal and adult skins. Results There were significant differences between the expression of homeohox genes, especially for PRX-2, HOXB13, HOXB6 and HOXB7. Conclusions The homeobox gene is in close relation to developmental hiology. The different expressions and changes of homeohox genes in the normal, wounded human fetal and adult skin may be a primal cause of different wound healing between fetal and aduh skin.