瘤性体液中肿瘤血管生长因子的提取和检测

Entracing and detecting of tumor angiogenesis factor in cancerous tumor

目的:建立科学有效的提纯、检测肿瘤血管生成因子(tumorangiogenesisfactor,TAF)的方法。方法:应用中空纤维超滤、超速离心、层析和电泳等方法,对肺癌及卵巢癌患者的胸腔积液、腹水中TAF进行提纯和分析;建立动物模型,采用鸡胚绒毛尿囊膜法检测其生物活性。结果:肺癌及卵巢癌患者的胸腔积液和腹水活性检测结果显示,87例中96.55%呈阳性,3.45%为阴性,差异有统计学意义。纯化的TAF经电泳后谱带清晰,相对分子质量为(4.6～4.8)×103,且具有生物活性,经免疫试验,抗体效价可达16.50。结论:所建立的提取、纯化及生物活性检测的方法稳定、有效,可用于观测抗肿瘤血管生成药物的药效或生物疗法的效果。肿瘤防治杂志,2005,12(17)

OBJECTIVE： To established a scientifically effective method for extracting and detecting the tumor angiogenesis factor （TAF） in cancerous tumor. METHODS： TAF was purified at scheduled intervals by using extract methods such as hollow fiber column, ultracentrifugation, chromatography and electrophoresis. A creaturely model was established that was used to monitor the biological activity by the method of chicken embryo villus allantoid. RESULTS： Tissue samples were obtained from splanchnocoeles of 87 sufferers with carcinoma of lung, carcinoma of ovary and the data were treated by statistics. For quantitative data, the biological activity of samples was measured or calculated at scheduled intervals. As a result, the level of biological activity increased significantly by 96. 55% positive and 4.45% negative. As been shown in gel electrophoresis after purification, the band of TAF was sharp and the molecular weight （4.6-4.8） × 10^3 was identified. Furthermore. positively biological activity was detected. Antihody titer was 16.50. CONCLUSIONS： The method of extracting and detecting TAF in cancerous humor is stable and effective.