丹参酮ⅡA对MKN-45细胞生长的影响

Effect of Tanshinone ⅡA on the growth of MKN-45 cell line in vitro

目的:研究丹参酮ⅡA(Tanshi-noneⅡA,TanⅡA)对人胃癌细胞株MKN-45的生长抑制作用。方法:选用人胃癌细胞株MKN-45,运用MTT法、流式细胞术(FCM)和逆转录聚合酶链式反应(RT-PCR)半定量法检测细胞增殖、细胞周期、细胞凋亡和各组p53mRNA表达。结果:TanⅡA可明显抑制MKN-45细胞的增殖,其抑制作用具有时间-效应和剂量-效应关系。当2·0μg/mL TanⅡA处理MKN-45细胞96h,增殖抑制率达(74·84±0·41)%。FCM检测肿瘤细胞DNA变化,观察到TanⅡA使MKN-45细胞周期阻滞于G2/M期,出现浓度依赖性的亚“G1”峰,同时野生型p53表达增加。结论:TanⅡA能有效地抑制人胃癌细胞株MKN-45的增殖,其可能的机制与凋亡有关。肿瘤防治杂志,2005,12(19)

OBJECTIVE： To investigate the effect of Tanshinone Ⅱ A on the growth of human gastric carcinoma cell line MKN- 45 in vitro. METHODS： Cell proliferation,cell cycle and apoptosis, the expression of p53 mRNA of cell line MKN-45 were detected by MTT assay, flow cytometry （FCM） and semiquantitative RT-PCR. RESULTS： The data showed that Tanshinone Ⅱ A effectively inhibited the proliferation of cell line MKN-45 in time- and concentrationdependent. The cell proliferation inhibition rate was （74.84 ± 0.41）% when treated with 2.0 μg/mL Tanshinone Ⅱ A for 96 hours. The change of DNA of MKN-45 cells indicated that Tanshinone Ⅱ A was able to block cell cycle progress in G2/M phase,with appearance of sub-G1 peak in a concentration- dependently. The expression of p53 was up-regulated in the process of Tanshinone Ⅱ A - inducing MKN-45 apoptosis. CONCLUSION： Tanshinone Ⅱ A can effectively inhibit the proliferation of human gastric carcinoma cell line MKN-45 in vitro and the possible mechanism may relate to modulation of the apoptosis associated gene expression, such as p53 up-regulated.