金雀异黄酮对成骨细胞增殖的影响及其可能的分子生物学机制

Effects of genistein on proliferation of osteoblast of post-menopausal patients and the likely molecular mechanisms

目的观察金雀异黄酮(GS)对绝经期骨质疏松患者骨膜成骨细胞增殖的影响及其分子生物学机制。方法用噻唑蓝比色法和流式细胞术分别检测GS对成骨细胞增殖活力和细胞周期分布的影响;Western-blot技术分析GS对细胞周期蛋白D和E表达的影响。结果GS可显著提高成骨细胞增殖活力,与溶剂对照组比较,10-8mol/L、10-7mol/L和10-6mol/LGS时,细胞增殖率分别为137%、155%和161%;提高S期和M/G2期细胞分布比例,并伴有G0/G1期细胞比例降低;它莫西芬可抑制10-7mol/L和10-6mol/LGS促成骨细胞增殖效应。Western-blot的检测结果显示,10-8mol/L和10-7mol/LGS对成骨细胞处理24h,提高细胞周期蛋白D和E蛋白质的表达(P<0.05)。结论由于雌激素受体拮抗剂它莫西芬可抑制GS对成骨细胞的促增殖作用,表明金雀异黄酮可通过激活雌激素受体途径,表现出雌激素效应,并促进骨组织代谢中的骨形成作用。

Objective This study was designed to explore the effects of phytoestrogen genistein on proliferation of post-menopausal osteoblast and the likely molecular mechanisms. Methods Osteoblast cultures were prepared from the upper femur of postmenopausal patients. Osteoblast proliferation was determined by Methyl Thiazolyl Tetrazolium（MTT）assay and cell cycle distribution by cytometry. The protein expressions of cyclin D and E were examined using Western-blot. Results Genistein （10^-8 to 10^-6 mol/L） stimulated cell viability of postmenopausal osteoblasts and increased the distribution ratio of the cells at the G2/M and S phases （P〈0.05） with a slight decrease in G0/G1 population. Osteoblasts demonstrated two- and fourfold increased cyclin D expression when treated with 10^-8mol/L （P〈0.05） and 10^-7mol/L （P〈0.01） GS, respectively, as compared with untreated cells. The changes of cyclin E expression at protein level were similar with cyclin D. Conclusions Taken together, these results suggested that flavonols genistein could stimulate osteoblast activity via an estrogen-receptor-dependent pathway. Such compounds may represent new pharmacological tools for the treatment of osteoporosis.