霍山石斛原球茎液体培养条件的优化

Optimization Conditions for Liquid Culture of Protocorm-like Bodies of Dendrobium Huoshanenes

为采用霍山石斛原球茎为原料,开发出高级保健型清音润喉饮品,本文以液体悬浮培养研究原球茎最适生长条件的优化。在单因素实验的基础上,选择了马铃薯汁、香蕉汁、NAA、IBA作四因素三水平正交试验,试验表明:(1)液体条件下霍山石斛原球茎适宜的基本培养基是1/2MS;(2)1/2MS培养基添加马铃薯汁100g/L、香蕉汁30g/L、IBA1.0mg/L组合,含蔗糖30g/L时,原球茎增殖倍数最大。正交试验结果验证显示:原球茎在40天内的增殖倍数可达12.56倍,多糖含量达8.30mg/g(鲜重)。

In order to exploit an advanced beverage for the health of human beings＇ throats from the protocorm-like bodies （PLBs） of medicinal Dendrobium Huoshanenes, the optimal conditions for PLBs culture are studied. On the basis of a single factor experiment, the orthogonal test is designed. Results showed that （1） 1/2MS was suitable for the growth of PLBs of Dendrobium Huoshanenes, （2） Combination of potato solution 100g/L, banana solution 30g/L, IBA 1.0mg/L and sucrose 30g/L in 1/2 MS was the best culture condition. When PLBs were cultured in optimized medium, proliferation of PLBs increased 12.56 times, and polysaccharide content was 8.30mg/gFW.