直投式乳酸菌发酵剂增菌培养基的优化

Optimization of Enrichment Culture of DVS Lactic-acid Bacteria Starter

为制备直投式乳酸菌发酵剂,本实验通过对碳氮源、营养因子及缓冲盐类进行优化实验,确定了嗜酸乳杆菌ATCC4356增菌培养基的配方为:在改良MRS培养基的基础上,添加10%番茄汁,10%胡萝卜汁,10%平菇汁,0.1g/100mlCaCO3,0.5g/100mlNaAc。将此菌在此增菌培养基上培养,在37℃下培养10h,菌数可达到8.56×109cfu/ml,与普通液体发酵剂相比,获得了显著的浓缩效果。

In order to prepare DLrect Vat Set （OVS） lactic-acid bacteria starter, me enrichment culture or Lactobacillus acidophilus ATCC 4356 was studied by orthogonality tests of carbon source, nitrogen source, nutrition factors and buffer salts. The optimum culture composition was as following： improved MRS culture, 10% tomato juice, 10% carrot juice, 10% juice of plerotus sapidus, 0.1 g/100ml CaCO3, 0.5g/100ml NaAc. The number of viable cells got to 8.56×10^9cfu/ml in the culture for 10 hours at 37℃. A notable effect of concentration was gained comparing with simple liquid starter.