生姜黄酮提取纯化及结构的初步鉴定

Study on Extraction Technology, Purification and Structural Appraisal of Flavonoid from Ginger

探讨了生姜黄酮的提取工艺、纯化方法及其可能的结构。结果表明生姜黄酮提取用40倍原料的90%甲醇溶液,在60～65℃条件下提取4h为其优化组合,而其试验组合中以用40倍原料的75%甲醇溶液,在60～65℃条件下提取2h的提取效果最好,最大得率是1.250%。在甲醇提取的生姜黄酮中,主要成分是双氢黄酮。通过对其紫外斑点检测、紫外光谱分析、颜色反应,初步判断可能是A环无邻位二羟基、无游离5-羟基、7-羟基的双氢黄酮。

Had studied the extraction technology purification method and structural appraisal of ginger＇s flavonoid. The results showed that the best excellent condition was adopting 40multiples＇ raw materials weight of 90% methyl alcohols solvent, current soaking 4h under 60-65℃. But the best combination was that adopting 40multiples＇ raw materials weight of 75% methyl alcohols solvent, current soaking 2h under 60-65℃. The highest obtain rate was 1.250%. The main composition is flavanones.