天花粉蛋白基因工程菌发酵条件研究

Fermentation Conditions for High-Level Expression of Trichosanthin Gene in Gene Engineering Strain

目的:通过对天花粉蛋白基因工程菌T239发酵条件研究,优化培养及表达条件。方法:研究温度、PH值、培养时间、培养基成分、接种量、诱导剂浓度等6种因素对于表达量的影响。结果:天花粉蛋白基因工程菌T239发酵条件在温度30℃、pH值7.3、摇床转速200r/min、50ml/500ml培养瓶、接种量3%、培养时间30h、添加葡萄糖浓度为2.0g/L时,表达量最高。

Objective： Engineering Strain（T239） was cultivated under different conditions in order to find the optimal Fermentations that would lead to maximal fusion protein yield. Methods： The strain was cultured under different temperature, pH, rotating rate, time, and capability of bottle. Results and conclusions： The proper Fermentation Conditions consist of： 30℃, pH7.3,200r/min, 50ml/500mlculture bottle, 30h, rotating cultivation bed, with 2.0g/L glucoseon added.