摘要
重金属离子镉与螯合剂IEDTA螯合后再与载体蛋白KLH偶联获得完全抗原(Cd-IEDTA-KLH),以该完全抗原免疫Balb/C小鼠,制备杂交瘤获得了稳定分泌抗Cd-ITCBA抗体的细胞株1F6。以该细胞株免疫小鼠腹腔,获得抗Cd-ITCBA的腹水型单克隆抗体,效价为1:51200。
Heavy metal ion cadmium was chelated with Isothiocyanobenzyl-EDTA （IEDTA） and linked with vector protein KLH to gain the completed antigen （Cd-IEDTA-KLH）. Several Balb/C mouse were immunized with Cd-IEDTA-KLH to make hybridoma cells. The strain 1F6 which can produce monoclonal antibody against Cd-IEDTA steadily was obtained. The ascites was obtained by immunized mouse with 1F6 cells. The titer of the monoclonal antibody in the ascites was about 1：51200.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第9期350-353,共4页
Food Science
基金
广东省科技计划面上项目(2003C20411)
关键词
重金属
镉
单克隆抗体
heavy metal
cadmium
monoclonal antibody
