玉米及其制品中转基因成分的单一PCR及多重PCR检测

Detection of Genetically Modified Components in Maize and Its Products by Single PCR and Multiplex PCR

采用CTAB法提取玉米及其制品的总DNA,用PCR方法检测其中的转基因成分如花椰菜花叶病毒(Cauliflowermosaicvirus,CaMV)35S启动子、根癌农杆菌(Agrobacteriumtumefaciens)胭脂碱合成酶基因(nos)终止子、根癌农杆菌CP4菌株的EPSPS基因、吸水链霉菌(Treptomyceshygroscopicus)bar基因及苏云金芽孢杆菌库尔斯塔克亚种(Bacillusthuringiensissubsp.kurstaki)cryIA(b)基因,筛选到阳性样品,并建立了几组玉米内源zein基因和转基因成分之间的多重PCR检测方法。结果表明,建立的多重PCR方法用于同时检测玉米内源基因和转基因成分是可行的,值得推广;虽然我国还未有已获准商品化生产的转基因玉米,但国外转基因玉米已流入福建省。

The total DNAs of maize and its products were extracted by CTAB method. The positive samples were screened by detecting of several genetically modified components such as Cauliflower mosaic virus（CaMV） 35S promoter, Agrobacterium tumefaciens nopaline synthase（nos） terminator, A.tumefaciens strain CP4 EPSPS gene, Treptomyces hygroscopicus bar gene and Bacillus thuringiensis subsp, kurstaki cryIA（b） gene with single PCR. The multiplex PCR methods detecting synchronously of maize endogenous zein gene and several genetically modified components were developed. The results showed that the multiplex PCR methods developed for detecting synchronously of endogenous gene and genetically modified components were practical and worthy of being popularized, and there is no commercial genetically modified maize in our country, but foreign commercial transgenic maizes were inpoured into fujian province.