海带脂肪酸邻氨基苯酚化学修饰气相色谱—质谱分析

Analysis of Fatty Acid in Laminaria Japonica by Gas Chromatography—Mass Spectrometry with Chemical Modifying Reagent Ortho—aminophenol

以邻氨基苯酚为脂肪酸的化学修饰试剂,气相色谱—EI质谱分析海带脂肪酸。邻氨基苯酚将脂肪酸羧基修改为含氮杂环,使在EI源中避免了链烯基中碳碳双键的移动。解析了海带脂肪酸邻氨基苯酚化学修饰产物的EI质谱图,讨论了烯酸中碳碳双键的定位规则,确定了海带脂肪酸中碳碳双键的位置。鉴定出海带脂肪酸中12种脂肪酸,其中不饱和脂肪酸相对含量为57.41%,多不饱和脂肪酸含量占34.01%。

A gas chromatography-electron impact mass spectrometry method was developed for analyzing the composition of the fatty acids from Laminaria japonica. Ortho-aminophenol was developed for chemical modifying reagent of fatty acids so as to modify carboxyl group into nitrogen-containing heterocycle which effectively suppresses the migration of double bonds of the alkenyl under the electron impact source. The empirical rule in which the position of double bonds of olefinic acids were located was refered to by analyzing election impact mass spectra of the chemical products modified by ortho-aminophenal of the fatty acids in Laminaria japonica. 12 fatty acids were identified according to the analysis of GC-EIMS and the position of double bonds of the fatty acids from Laminaria japonica was located. The contents of the unsaturated fatty acids and the polyunsaturated fatty acids are 57.41% and 34.01%.