龙须菜藻胆蛋白免疫功能和抗氧化作用的研究

Study on the Immunity Function and Anti-oxidation Activity of Phycobiliproteins from Gracilaria Lemaneiformis

目的探讨龙须菜藻胆蛋白粗提物对小鼠免疫功能和抗氧化能力的影响。方法以硫酸铵沉淀法提取龙须菜藻胆蛋白粗提物,经口给予小鼠100、200、300(mg/kg·d)的藻胆蛋白粗提物,连续灌胃15和30d后,分别测定小鼠腹腔巨噬细胞的吞噬能力及小鼠脾、胸腺指数和血红细胞SOD活性及血清过氧化脂质(LPO)含量。结果藻胆蛋白粗提物能有效增强小鼠腹腔巨噬细胞的吞噬能力,刺激小鼠脾脏和胸腺器官的发育;且能极显著地提高血红细胞SOD的活性,降低LPO含量。结论龙须菜藻胆蛋白粗提物具有提高小鼠免疫能力和抗氧化作用。

Objection： To investigate the immunity function and anti-oxidation activity of phycobiliproteins from Gracilaria Lemaneiformis in mice. Methods： The mice was treated by Gastric incubation with the crude phycobiliproteins, which were got from Gracilaria Lemaneiformis by ammonium sulfate precipitation, at doses of 100. 200. 300mg/kg/d .The phagocytosing function of celiac macrophage, the spleen index and the thymus index, the activity of erythrocyte superoxide dismutase （SOD） and the content of serum lipoid peroxide （LPO） in mice respectively were determined on the 15th day and 30th day of the treatment. Result： The crude phycobiliprotens could significantly improve the phagocytosing function of celiac macrophage, and stimulate the growth of spleen and thymus; and also can increased the activity of erythrocyte SOD and decreased the content of LPO significantly. Conclusion： The crude phycobiliproteins from Gracilaria Lemaneiformis has the abilities to increasing the immunity function and anti-oxidation activity in mice.