利用外周血象指标评价耐缺氧作用的方法研究

Study on a Method of Measuring the Periphery Blood Targets of Mice on Anti-hypoxia Effect

为了减少动物实验次数和受试小鼠的使用数量,进而减少因小鼠个体差异而造成的误差,提高耐缺氧功效评价结果的准确性和重现性,本文着重探讨了利用外周血象指标评价耐缺氧功效的方法研究。实验发现:不同受试天数、不同停留时间对不同实验组受试小鼠血象中红细胞(RBC)、血红蛋白(HB)、红细胞压积(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白量(MCH)、平均红细胞血红蛋白浓度(MCHC)六项指标的影响情况是不同的。若仅以受试30d或15d的小鼠外周血象中个别指标的变化情况评价其耐缺氧特性具有一定的偶然性。因此,评价时应该研究不同受试天数、不同停留时间对受试小鼠外周血象指标的影响情况进行综合分析,才能保证功效评价结果准确和客观。

In order to decrease experiment times and the amount of tested mice, to decrease error caused by the difference of individual mouse, to decrease the fortuitous factors of functional evaluation, and overcome dissimilarity of the appearance of result, we did some animal experiments. The result of measuring the periphery blood targets of mice shows： different experiment time and stay time caused different results in Erythrocyte（RBC）, Hemoglobin（HB）, The bulk of condensation of the erythrocyte （HCT）, Equally erythrocyte bulk（MCV）, Equally the erythrocyte hemoglobin（MCH）, Equally erythrocyte hemoglobin concentration（MCHC）.There will be some fruits if the alterable situation of the periphery blood targets of mice, which tested only 30days or 15days, were used in evaluating their anti-hypoxia. Therefore, In order to get accurate and objective evaluating results, the periphery blood targets of mice should be synthetically analyzed by different experiment time and stay time.